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51.
Jennifer S. Taintor DVM Terri HathcockMarta Barba DVM Fernanda Cesar Heather Gossett 《Journal of Equine Veterinary Science》2014
This pilot study was performed to determine the need for future investigation of the effect of transportation, environmental changes, and athletic competition on fecal shedding of Salmonella spp. in sport horses. Fecal samples were collected once daily from 20 healthy horses that were competing in National Collegiate Athletic Association equestrian event that occurred over a 3-day period. The horses were divided into four groups with five horses per group. The groups were divided based on travel distance. Fresh fecal samples were collected daily and cultured for Salmonella spp., through bacterial culture, for a minimum of 3 days for three groups that traveled to compete and for 15 days for a group that was housed at the competition location. All fecal samples were negative for Salmonella spp. Although this was a pilot study with a small sample size and limited collection days, the results of this study question the effects of the stress of transportation, new environment, and athletic activity on fecal shedding of Salmonella spp. in horses. A larger study, however, is needed to further investigate these findings. 相似文献
52.
Davidson EH Rast JP Oliveri P Ransick A Calestani C Yuh CH Minokawa T Amore G Hinman V Arenas-Mena C Otim O Brown CT Livi CB Lee PY Revilla R Rust AG Pan Zj Schilstra MJ Clarke PJ Arnone MI Rowen L Cameron RA McClay DR Hood L Bolouri H 《Science (New York, N.Y.)》2002,295(5560):1669-1678
Development of the body plan is controlled by large networks of regulatory genes. A gene regulatory network that controls the specification of endoderm and mesoderm in the sea urchin embryo is summarized here. The network was derived from large-scale perturbation analyses, in combination with computational methodologies, genomic data, cis-regulatory analysis, and molecular embryology. The network contains over 40 genes at present, and each node can be directly verified at the DNA sequence level by cis-regulatory analysis. Its architecture reveals specific and general aspects of development, such as how given cells generate their ordained fates in the embryo and why the process moves inexorably forward in developmental time. 相似文献
53.
Zabalza A González EM Arrese-Igor C Royuela M 《Journal of agricultural and food chemistry》2005,53(19):7486-7493
The inhibition of branched-chain amino acid (BCAA) biosynthesis was evaluated in pea plants in relation to the ability for induction of fermentative metabolism under aerobic conditions. Chlorsulfuron and imazethapyr (inhibitors of acetolactate synthase, ALS, EC 4.1.3.18) produced a strong induction of pyruvate decarboxylase (PDC, EC 4.1.1.1) and alcohol dehydrogenase (ADH, EC 1.1.1.1) activities and a lesser induction of lactate dehydrogenase (LDH, EC 1.1.1.27) and alanine aminotransferase (AlaAT, EC 2.6.1.2) activities in roots. Inhibition of the second enzyme of the BCAA biosynthesis (ketol-acid reductoisomerase, KARI, EC 1.1.1.86) by Hoe 704 (2-dimethylphosphinoyl-2-hydroxyacetic acid) and CPCA (1,1-cyclopropanedicarboxylic acid) enhanced fermentative enzyme activities including PDC, ADH, and AlaAT. Fermentative metabolism induction occurring with ALS- and KARI-inhibitors was related to a higher expression of PDC. In the case of KARI inhibition, it is proposed that fermentation induction is due to an inhibition of ALS activity resulted from an increase in acetolactate concentration. Fermentative metabolism induction in roots, or at least ethanolic fermentation, appeared to be a general physiological response to the BCAA biosynthesis inhibition. 相似文献
54.
Leite da Silva Wilian Aparecido Poehland Ralf Carvalho de Oliveira Caroline Ribeiro Ferreira Mariane Gabriela Cesar Garcia de Almeida Ricardo Cáceres Mirela Brochado Souza Macedo Gustavo Guerino da Costa e Silva Eliane Vianna Alves Fabiana Villa Nogueira Eriklis de Andrade Melo-Sterza Fabiana 《Tropical animal health and production》2020,52(5):2273-2281
Tropical Animal Health and Production - The aim of this study was to evaluate the impact of increased shadow supply in integrated crop-livestock-forest systems on in vitro embryonic development and... 相似文献
55.
56.
- This study aimed to determine biological and distributional aspects of deep-sea giant isopods Bathynomus giganteus and Bathynomus miyarei to assess their extinction risk.
- Bathynomus giganteus (663 specimens) and B. miyarei (649 specimens) were collected during five research cruises involving 265 h of sampling effort during 32 fishing operations of circular pots, baited traps, ranging from 400 and 1,000 m deep, between 26 and 29°S adjacent to the Brazilian coast.
- A trend towards larger, sexually mature animals in shallower regions was observed for both species, and depth was the most important environmental variable influencing the animals’ distribution.
- Bathynomus giganteus were collected between 600 m and 1,000 m depth, while the highest densities of B. miyarei were at shallower depths, between 400 and 600 m.
- The sex ratio was equal for both B. giganteus and B. miyarei for different seasons, latitudes and bathymetries.
- The sexual maturity of B. giganteus was estimated at 340–345 mm total length for males and between 280–290 mm for females. Bathynomus miyarei males’ sexual maturity was estimated in the 225–230 mm size class.
- Bathynomus giganteus showed reproductive activity throughout the year.
- The longevity of B. giganteus was estimated at 6 years for males and 7.7 years for females. The longevity of B. miyarei was estimated at 9 years for males and 6 years for females.
- Extinction risk assessment for B. giganteus, which has a greater latitudinal and bathymetric distribution, was evaluated as Least Concern. However, B. miyarei was evaluated in the Data Deficient category.
57.
Gerson Laerson DRESCHER Leandro Souza DA SILVA Qamar SARFARAZ Gustavo DAL MOLIN Laura Brondani MARZARI Augusto Ferreira LOPES Cesar CELLA Daniela Basso FACCO Rodrigo Knevitz HAMMERSCHMITT 《土壤圈》2020,30(3):326-335
Nitrogen (N) is a key nutrient for rice production, and its bioavailability in paddy soils is strongly coupled to soil organic matter (SOM) cycling. A better understanding of potentially available N forms in soil, such as alkaline hydrolyzable N (AH-N), and their depth distribution will support the development of best management practices to improve the N use efficiency of rice while minimizing adverse environmental effects. Fifteen rice (Oryza sativa L.) fields from Southern Brazil were selected, and stratified soil samples were taken to a depth of 60 cm before crop establishment. Selected soil physical and chemical properties were analyzed to evaluate their relationships with AH-N contents in the soil profile. The AH-N contents below 20 cm varied extensively (increased, reduced, or constant) compared with that above 20 cm. Although clay and clay + silt contents were highly correlated to AH-N for some soils, the major property dictating AH-N distribution by depth was total N (TN), as the correlation between TN and AH-N was mainly by direct effect. The proportion of TN recovered as AH-N across sites and depths presented high amplitude, and thus AH-N was not a constant N pool across depths, indicating that AH-N can be affected by soil management practices even when TN showed no major changes. The distinct distribution of AH-N across soil sampling sites and depths indicates that depths greater than 20 cm should be considered when calibrating the AH-N index for N fertilizer recommendations for flooded rice in Southern Brazil. 相似文献
58.
59.
Camila Pegoraro Liliane Marcia Mertz Luciano Carlos da Maia Cesar Valmor Rombaldi Antonio Costa de Oliveira 《Journal of Crop Science and Biotechnology》2011,14(2):85-95
Abiotic and biotic stress conditions cause extensive losses to maize production, mainly due to protein dysfunction in these
conditions. In higher plants, the occurrence of heat-shock proteins (HSPs) in response to different environmental stresses
is a universal phenomenon and has been well documented. Many studies have demonstrated that most HSPs are involved in many
regulatory pathways, act as molecular chaperones for other cell proteins, and have strong cytoprotective effects. Although
many functional roles for HSPs are known, the mechanisms for these multiple functions are not entirely understood. Here we
reviewed the correlation among HSP genes/proteins and plant tolerance, especially maize, in different environmental stresses. Due to the low availability of
information regarding the expression of HSP genes in response to different stresses in maize, we decided to mine databases in order to generate new insights related
to this topic. 相似文献
60.
Manthey JA Cesar TB Jackson E Mertens-Talcott S 《Journal of agricultural and food chemistry》2011,59(1):145-151
Nobiletin (NOB) and tangeretin (TAN), two of the main polymethoxylated flavones (PMFs) in citrus, influence a number of key biological pathways in mammalian cells. Although the impacts of NOB and TAN on glucose homeostasis and cholesterol regulation have been investigated in human clinical trials, much information is still lacking about the metabolism and oral bioavailability of these compounds in animals. In this study, NOB and TAN were administered to rats by gavage and intraperitoneal (ip) injection, and the blood serum concentrations of these compounds and their main metabolites were monitored by high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). In addition to the administered compounds, two metabolites of TAN and eight metabolites of NOB were detected and measured over 24 h. With identical oral doses, nearly 10-fold higher absorption of NOB occurred compared to TAN. For both compounds, maximum levels of glucuronidated metabolites occurred in the blood serum at later time points (~5-8 h) compared to the earlier T(max) values for NOB and TAN. In most cases the glucuronides occurred at substantially higher concentrations than the aglycone metabolites. Low levels of NOB and TAN and their metabolites were detectable in rat blood serum even at 24 h after treatment. 相似文献